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Find Chimeras
Design Primers
Design Probes
Design Array
16S Oligos
You can use DECIPHER's Design Primers web tool to design the optimal set of PCR primers for targeting one group of DNA sequences in the presence of many non-target groups. If you are looking for genus-specific 16S primers then also try our 16S Oligos tool.

F.A.Q.        Inputs        Outputs

Email address (where to send results): [?]

Job title (optional): [?]

Use Taq 3'-end Model to improve specificity?: [?]
No Yes Yes and induce a mismatch

Primers: [?] Length - nucleotides with up to permutation(s)

PCR Product Amplicon: [?] Length - base pairs

Reaction Conditions: [?]
[Na+] = mM,  [Mg++] = mM,  [dNTPs] = mM
Annealing Temperature = °C,  [Primers] = nM

Target group: [?] with at least % coverage

Choose aligned FASTA file (limit 50 MB): [?]