Find Chimeras - Outputs:
The results are sent to the e-mail address provided by the user. The e-mail includes a text message that contains the chimeras that were found (text given in the description line of the FASTA entries). The detailed tab-delimited text attachment shows the results in detail, using a table of six columns. After unzipping the file, the user can open this file with a spreadsheet program, such as MS Excel, for analyzing the results. The titles and contents of the output columns are as follows:
The given description of the sequence in the FASTA-formatted input file.
Taxonomic classification of the sequence based on the RDP MultiClassifier program (at 80% confidence level).
The name of the group in DECIPHER’s database, to which the sequence was assigned during the chimera test (based on a 51% confidence of classification).
The number of non-gap characters, including ambiguity codes, in each sequence.
One of these three different outcomes: “Not deciphered as a chimera”, “Chimera”, or “Indecipherable”. The latter outcome indicates that the sequence was not evaluated since it could not be assigned to a group that meets the criteria for finding chimeras. Often indecipherables are sequences that are chimeric or are not 16S.
When a sequence is a putative chimera, this column indicates the group of the potential parents of the chimeric fragment, followed by the position of the fragment in the sequence (5’ and 3’ ends; numbering according to the original sequence without gaps). Otherwise, the chimeric region output is “NA”, meaning not applicable.